Characterization of cell-material interactions

In vivo – which means in the natural surrounding tissue – cells are to be found in a structure, the so-called extracellular matrix, with which they communicate and interact by means of various stimuli. Different characteristics of this cell environment such as mechanical properties, charge distributions or the topography have a direct influence on the cell behavior. Biomaterials that reflect these nature-given properties to a high degree, are the basis for functional, durable and compatible products.

Primary keratinocytes on nubbed structure. The cell interacts directly with the nubs and is firmly anchored.

Human endothelial cell, grown on a 3D-printable polymer.

Our knowledge of the interactions between cells and materials as well as our expertise in the field of cell cultivation enable us to identify the influence of the chemical composition or various surface structures of the biomaterials on cells. In addition to standardized analytical processes we also develop new methods of analysis to meet your requirements.

Tailored to the intended use of the product, for the cell type that is relevant to the application in question, we can evaluate the following parameters on the biomaterial:

  • Cell morphology
  • Cell adhesion:
    • formation of cell-cell junctions
    • formation of the actin skeleton
  • Cell-matrix interaction:
    • number/character of filopodia
    • cell alignment
  • Cell-differentiation:
    • on the protein level
    • immunohistochemical analyses
    • evidence of released factors (e.g. inflammation mediators)
    • on the RNA level
  • Hemocompatibility:
    • coagulation
    • hemolysis, where applicable complement activation
  • Proliferation capability
  • Cell-vitality
  • Cell-damage:
    • morphological changes
    • membrane disintegrity
  • Cell-specific parameters:
    • enzyme activity