Functional Genomics

Next Generation Sequencing Services

The Functional Genomics group has established a three-step process to handle inquiries for high throughput sequencing. This process, consisting of sample preparation, next generation sequencing and bioinformatics, is highly adaptive due to various established preprocessing and sample preparation protocols, as well as fast and flexible bioinformatics.

These characteristics enable a variety of applications, like de-novo genome and transcriptome sequencing of biotechnological production strains or human pathogens or meta-genomics and meta-transcriptomics, as well as facilitating early diagnosis of infectious and cancerous diseases.

Established NGS-technologies 

  Read length Reads/
Flowcell
Capacity/
Flowcell
Advantages
Illumina HiSeq2500
(HighOutput)
2x100 Bases 3,2 bn 300 Gb wide variety of applications,
high throughput
Illumina HiSeq2500
(Rapid)
2x250 Bases 600 Mio 150 Gb fast data generation

 

Equipment

QIAcube (Qiagen)

  • automated sample preparation using Qiagen kits
  • up to 12 samples

 

Axio Observer.Z1 microscope (Zeiss)

  • ApoTome
  • fluorescence microscopy

 

BioAnalyzer 2100 (Agilent)

  • quality control, RNA and DNA quantification

 

Biomek Fx (Beckman Coulter)

  • automated sample preparation
  • individually programmable protocols

 

GS Junior (Roche)

  • pyrosequencing with long read length of up to 700 bp

 

HiSeq2500 (Illumina)

  • high throughput sequencing 
  • various kits for sample preparation to address different scientific problems

 

HPC

  • high-performance computing cluster with four computing units (100 CPUs, 770GB RAM)
  • backup via on-site tape library