Cell-based Assays

Services

  • Fast and easy qualitative and quantitative detection of pyrogens e.g. in medical devices, such as surgical instruments, catheters, implants and also intravenously administered drugs.
  • Screening for new agonists and antagonists of the immune receptors
  • Detection of individual ligands or combination of ligands with isolated PRRs

Reference projects

Screening for new immunmodulators – Discovery and Delivery of PRR antagonists and agonists to regulate innate immune reaction

Project duration: November 2012 – October 2015

The receptors of the innate immune system recognize not only conserved molecular patterns of infectious pathogens, but also isolated chemical structures (PAMPs, pathogen-associated molecular patterns), and are referred to as pattern recognition receptors (PRRs).

more info

Detection of pyrogens in purified human IgG (polyclonal) samples using a specific cell-based reporter gene assay for: Talecris Biotherapeutics Inc. 8368 US 70 West, Clayton, North Carolina 27520, USA

Publications

  1. Burger-Kentischer, A., Abele, I. S., Finkelmeier, D., Wiesmuller, K. H., and Rupp, S. (2010): A new cell-based innate immune receptor assay for the examination of receptor activity, ligand specificity, signalling pathways and the detection of pyrogens, J Immunol Methods, 358: 93-103 (2010).