Non-Invasive Analysis of Cells and Tissues

Cell and tissue analysis for basic research, tissue engineering, regenerative medicine and test systems

The isolation and culture of cells from primary material (various tissues and species), differentiation and proliferation of pluripotent stem cells, construction of three-dimensional cell culture systems and the fields of tissue engineering and regenerative medicine are the expertise of the Fraunhofer IGB. We characterize cells with classical invasive methods such as histological and immuno-histological staining, but we have developed and adopted numerous non-invasive methods that allow for the continuous monitoring of cell culture and tissue on the molecular and morphological level. With over 20 years experience in cell culture, we are your partner for complex questions in regenerative medicine, tissue engineering and the development of cell-based assays for toxicology and biocompatibility.

Fields of Research

Raman spectroscopy

Raman spectroscopy has been a standard tool for pharmaceutical quality control for a number of decades. Recently, Raman has seen a rebirth in the field of biomedical technology. The ability to non-invasively create a molecular fingerprint of cell and tissue without altering their state is of great value for both scientists and clinicians. At the Fraunhofer IGB, we have developed a well-established and published expertise in the Raman analysis of cells and extracellular matrix proteins, as well as other biologicals such as bacteria and cytotoxicity.

Multiphoton Imaging

Powerful laser technologies have enabled the development of new non-invasive analysis techniques such as second harmonic generation, fluorescence lifetime imaging, imaging flow cytometry and single photon counting. Our scientists have developed confocal laser methodologies that are considered the gold standard in the pharmaceutical industry. With our partner lab at the University Hospital Tübingen, we offer cutting edge cell analysis services for both industry and academic projects.

Laser microdissection

Automated microdissection allows the cutting of the smallest areas out of the matrix-immobilized cell layer. Even individual cell nuclei can be isolated for further molecular-biological analysis.

Flow cytometry - FACS sorting and characterization

Flow cytometry and imaging flow cytometry allow for the analysis and sorting of heterogeneous cell populations with antibody-coupled fluorescence dyes. Areas of application range from blood diagnostics, via cell transfection for the generation of cell lines and clone isolation to the identification of stem and precursor cell populations from a great variety of tissue types.