Algae biotechnology

Photosynthesis is the only bioprocess capable of building biomass using sunlight as an energy source and carbon dioxide as a carbon source. Microalgae are particularly well-suited for a material or energetic utilization of photosynthetically produced biomass, since the unicellular phototrophic organisms can utilize sunlight more efficiently than higher plants. Microalgae produce a variety of basic chemical substances such as polysaccharides, fatty acids, proteins, vitamins, and carotenoids with a high value-added potential for the pharmaceutical, food, feed, agricultural, and cosmetics industries, as well as biofuels and platform chemicals for biopolymers and textiles.

Processes for the production of biomass and valuable substances from microalgae

Fraunhofer IGB develops processes for the tailor-made production of a wide variety of products from microalgae and cyanobacteria in flat-plate airlift reactors. Through targeted process control, functional ingredients can be produced in high concentrations up to the pilot scale. To increase added value from algal biomass, we have investigated methods for extracting various functional compounds from algal biomass, such as carotenoids like fucoxanthin and astaxanthin, the omega-3 fatty acid EPA as a polar glycolipid, proteins and triglycerides for the production of food, feed, cosmetics, agrochemicals, biopolymers, and as feedstocks for bio-based polymers. For this purpose, we also employ new downstream processing technologies, such as the extraction of carotenoids and omega-3 fatty acids, cell disruption using pressure-change technology, and extraction with sub- or supercritical fluids.

Proprietary Flat-Panel Airlift Photobioreactor (FPA-PBR)

With its proprietary Flat-Panel Airlift Photobioreactors (FPA-PBR), Fraunhofer IGB provides a technology for the commercial-scale production of algal biomass with outstanding productivity, product quality, and cost efficiency. The reactors are modularly scalable (low-risk) and can be deployed on-site (robust, automated, remote maintenance) as a drop-in technology. This accelerates the transfer of new products.

Closing material cycles: CO2 and nutrient utilization by microalgae

Microalgal production can also be utilized as a unit operation in biorefineries, based on the principle of bioeconomic value chains, to utilize exhaust CO2 (carbon capture and use) and nutrients from wastewater streams and liquid fermentation residues, thereby closing material cycles through industrial biotechnological processes.

Advantages and unique selling points

Benefits of using our technology and partnering with us:

  • Market-ready technology and IP
  • Ethically sound products with high customer acceptance (climate-friendly, vegan, healthy)
  • Extraction of various valuable materials from algal biomass via cascaded extraction
  • Industrial transformation: targeted response with innovative processes and new products through process electrification, CO2 emissions trading, raw material resilience, material recovery, local production, no transportation, low land and water consumption, climate-independent agricultural production
 

Flat-panel airlift photobioreactor

Our photobioreactor, optimized through years of development work, features artificial lighting and is modularly scalable. Thanks to comprehensive automation, it can be operated on-site via remote maintenance. The system is available for the development of processes for microalgae cultivation and for the production of pilot-scale batches.

 

Microalgae products

We develop single- or two-stage production processes for the production of valuable substances such as omega-3 fatty acids (EPA), carotenoids like fucoxanthin, lutein, or astaxanthin, as well as protein-rich algal biomass or storage products such as oil and starch for energy use. For the processing of these substances, we develop efficient extraction methods and provide sample quantities for further analysis.

 

Algae cultivation for nutrient removal and as a CCU system

Algae’s ability to absorb CO2 and convert it into biomass, storage materials, and valuable resources makes them suitable for use in carbon capture and utilization (CCU) systems. In addition, algae can be cultivated using nutrient-rich wastewater streams, thereby purifying them. The use of microalgae for bioeconomic biorefinery value chains has been demonstrated in various projects.

  • Screening for microalgae and cyanobacteria with a wide variety of properties and constituents
  • Development of photoautotrophic processes from laboratory to pilot scale for microalgae and cyanobacteria in flat plate airlift reactors
  • Process optimization to improve productivity and biomass yield also using off-gas COand liquid fermentation residues
  • Development and adaptation of control software for algae production in the field
  • Development of processes for the isolation, fractionation and purification of algal constituents with the fullest possible utilization of the various fractions
  • Contract production of algae biomass with defined composition on a 100 kg scale

Infrastructure and equipment

Algae cultivation

  • Fully automated pilot plant for the cultivation of microalgae (greenhouse and field) based on flat-panel airlift reactor technology.
    • Indoor (greenhouse): five modules with five 6-liter reactors each, five modules with five 30-liter reactors each, three modules with five 180-liter reactors each - Total of 65 reactors with 3.6 m³ capacity
    • Outdoor (open air): one module with five 180-L reactors, four modules with ten 180-L reactors each - Total of 45 reactors with 8.1 m³ capacity

 

Brochure “Algae - Sustainable source of raw materials for materials and energy”

Scientific publications

JAHR
YEAR
TITEL/AUTOR:IN
TITLE/AUTHOR
PUBLIKATIONSTYP
PUBLICATION TYPE
2022 Management of Hypercholesterolemia Through Dietary ß-glucans-Insights From a Zebrafish Model
Gora, A.H.; Rehman, S.; Kiron, V.; Dias, J.; Fernandes, J.M.O.; Olsvik, P.A.; Siriyappagouder, P.; Vatsos, I.; Schmid-Staiger, Ulrike; Frick, K.; Cardoso, M.
Zeitschriftenaufsatz
Journal Article

 

2022 Tocochromanol Profiles in Chlorella sorokiniana, Nannochloropsis limnetica and Tetraselmis suecica Confirm the Presence of 11′-α-Tocomonoenol in Cultured Microalgae Independently of Species and Origin
Montoya-Arroyo, A.; Lehnert, K.; Muñoz-González, A.; Schmid-Staiger, Ulrike; Vetter, W.; Frank, J.
Zeitschriftenaufsatz
Journal Article

 

2022 The Microalgae Phaeodactylum tricornutum is Well Suited as a Food with Positive Effects on the Intestinal Microbiota and the Generation of SCFA: Results from a Pre-Clinical Study
Stiefvatter, L.; Neumann, U.; Rings, A.; Frick, Konstantin; Schmid-Staiger, Ulrike; Bischoff, S.C.
Zeitschriftenaufsatz
Journal Article

 

2021 Immune Status and Hepatic Antioxidant Capacity of Gilthead Seabream Sparus aurata Juveniles Fed Yeast and Microalga Derived v-glucans
Reis, B.; Goncalves, A.T.; Santos, P.; Sardinha, M.; Conceicäo, L.E.C.; Serradeiro, R.; Perez-Sanchez, J.; Calduch-Giner, J.; Schmid-Staiger, U.; Frick, K.; Dias, J.; Costas, B.
Zeitschriftenaufsatz
Journal Article

 

2021 Oral Bioavailability of Omega-3 Fatty Acids and Carotenoids from the Microalgae Phaeodactylum tricornutum in Healthy Young Adults
Stiefvatter, L.; Lehnert, K.; Frick, K.; Montoya-Arroyo, A.; Frank, J.; Vetter, W.; Schmid-Staiger, U.; Bischoff, S.C.
Zeitschriftenaufsatz
Journal Article

 

2020 Coproduction of EPA and Fucoxanthin with P. tricornutum - A Promising Approach for Up‐ and Downstream Processing
Derwenskus, Felix; Schäfer, Benjamin; Müller, Jan; Frick, Konstantin; Gille, Andrea; Briviba, Karlis; Schmid-Staiger, Ulrike; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2020 Prozessentwicklung zur Coproduktion von Eicosapentaensäure und Fucoxanthin mit der Kieselalge Phaeodactylum tricornutum
Derwenskus, Felix; Weickert, Sebastian; Frick, Konstantin; Schmid-Staiger, Ulrike; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2020 Verfahren sowie Vorrichtung zum Aufbereiten von Zellsuspensionen
Egner, Siegfried; Solyom, Katalin; Vasquez, Ana Lucia; VaronaIgleisias, Salima; Schmid-Staiger, Ulrike
Patent

 

2020 Economic evaluation of up- and downstream scenarios for the co-production of fucoxanthin and eicosapentaenoic acid with P. tricornutum using flat-panel airlift photobioreactors with artificial light
Derwenskus, Felix; Weickert, S.; Lewandowski, I.; Schmid-Staiger, U.; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2020 Verfahren zum Erhalt von Fucoxanthin und Fettsäuren aus Algenbiomasse
Derwenskus, Felix; Schmid-Staiger, Ulrike; Bringmann, Christian
Patent

 

2019 Pressurized extraction of unsaturated fatty acids and carotenoids from wet Chlorella vulgaris and Phaeodactylum tricornutum biomass using subcritical liquids
Derwenskus, Felix; Metz, Felix; Gille, Andrea; Schmid-Staiger, Ulrike; Briviba, Karlis; Schließmann, Ursula; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2019 Fucoxanthin, a carotenoid derived from Phaeodactylum tricornutum exerts antiproliferative and antioxidant activities in vitro
Neumann, U.; Derwenskus, Felix; Flister, V.F.; Schmid-Staiger, U.; Hirth, Thomas; Bischoff, S.C.
Zeitschriftenaufsatz
Journal Article

 

2019 A lipophilic fucoxanthin-rich Phaeodactylum tricornutum extract ameliorates effects of diet-induced obesity in C57BL/6J mice
Gille, Andrea; Stojnic, Bojan; Derwenskus, Felix; Trautmann, Andreas; Schmid-Staiger, Ulrike; Posten, Clemens; Briviba, Karlis; Palou, Andreu; Bonet, M. Luisa; Ribot, Joan
Zeitschriftenaufsatz
Journal Article

 

2019 Organismen als Produzenten: Produktion von Wertstoffen mithilfe von Mikroorganismen, Algen und Pflanzenzellen
Rasche, Stefan; Schillberg, Stefan; Derwenskus, Felix; Schmid-Staiger, Ulrike; Schließmann, Ursula
Aufsatz in Buch
Book Article

 

2019 Outdoor microalgae cultivation at different biomass concentrations - Assessment of different daily and seasonal light scenarios by modeling
Holdmann, Claudia; Schmid-Staiger, Ulrike; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2018 Bioavailability and safety of nutrients from the microalgae Chlorella vulgaris, Nannochloropsis oceanica and Phaeodactylum tricornutum in C57BL/6 mice
Neumann, Ulrike; Derwenskus, Felix; Gille, Andrea; Louis, Sandrine; Schmid-Staiger, Ulrike; Briviba, Karlis; Bischoff, Stephan C.
Zeitschriftenaufsatz
Journal Article

 

2018 Anti-inflammatory effects of Phaeodactylum tricornutum extracts on human blood mononuclear cells and murine macrophages
Neumann, Ulrike; Louis, Sandrine; Gille, Andrea; Derwenskus, Felix; Schmid-Staiger, Ulrike; Briviba, Karlis; Bischoff, Stephan C.
Zeitschriftenaufsatz
Journal Article

 

2018 Keeping the light energy constant - cultivation of Chlorella sorokiniana at different specific light availabilities and different photoperiods
Holdmann, Claudia; Schmid-Staiger, Ulrike; Hornstein, Helena; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2017 Primary Production
Lewandowski, I.; Lippe, M.; Montoya, J.C.; Dickhöfer, U.; Langenberger, G.; Pucher, J.; Schließmann, U.; Schmid-Staiger, U.; Derwenskus, Felix; Lippert, C.
Aufsatz in Buch
Book Article

 

2016 Kultivierung von Chlorella sorokiniana SAG 211/8k bei verschiedenen Lichtintensitäten und unterschiedlichen Beleuchtungszyklen
Holdmann, Claudia; Schmid-Staiger, Ulrike; Hornstein, Helena; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2016 Mikroalgenbioraffinerie - Entwicklung von Zellaufschluss- und Extraktionsprozessen zur Kaskadennutzung von Mikroalgenbiomasse
Derwenskus, Felix; Schmid-Staiger, Ulrike; Schließmann, Ursula; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2016 Microalgae - Underestimated All-rounders
Holdmann, Claudia; Derwenskus, Felix
Zeitschriftenaufsatz
Journal Article

 

2016 Kaskadenextraktion von Mikroalgenbiomasse zur Gewinnung von Wertstoffen für die Lebensmittelproduktion
Derwenskus, Felix; Metz, Felix; Hardtmann, Matthias; Schmid-Staiger, Ulrike; Schließmann, Ursula; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2015 First developments towards closing the nutrient cycle in a biofuel production process
Bagnoud-Velásquez, M.; Schmid-Staiger, U.; Peng, G.; Vogel, F.; Ludwig, C.
Zeitschriftenaufsatz
Journal Article

 

2013 Optimization of outdoor cultivation in flat panel airlift reactors for lipid production by Chlorella vulgaris
Münkel, R.; Schmid-Staiger, U.; Werner, A.; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2013 Hybrid life support systems with integrated fuel cells and photobioreactors for a lunar base
Belz, S.; Ganzer, B.; Messerschmid, E.; Friedrich, K.A.; Schmid-Staiger, U.
Zeitschriftenaufsatz
Journal Article

 

2012 Optimierung des Lipidproduktionsprozesses mit Chlorella vulgaris im Freiland hinsichtlich Lichtausbeute und Lipidgehalt
Münkel, R.; Schmid-Staiger, U.; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2012 Untersuchung des Zellaufschlusses von Phaeodactylum tricornutum
Schmid-Staiger, U.; Groeger, C.; Seibert, A.; Schliessmann, U.; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2009 Kultivierung von Mikroalgen im Photobioreaktor zur stofflichen und energetischen Nutzung
Schmid-Staiger, U.; Preisner, R.; Marek, P.; Trösch, Walter
Zeitschriftenaufsatz
Journal Article

 

2009 CO2-Killer und Rohstofflieferant - Kultivierung und Nutzung von Mikroalgen
Seibert, A.; Schmid-Staiger, U.; Trösch, Walter; Hirth, Thomas
Zeitschriftenaufsatz
Journal Article

 

2005 PHOTOBIOREAKTOR MIT VERBESSERTEM LICHTEINTRAG DURCH OBERFLAECHENVERGROESSERUNG, WELLENLAENGENSCHIEBER ODER LICHTTRANSPORT
Trösch, W.; Schmid-Staiger, Ulrike; Zastrow, A.; Retze, A.; Brucker, F.
Patent

 

2004 Optimization of eicosapentaenoic acid production by Phaeodactylum tricornutumin the flat panel airlift (FPA) reactor
Meiser, A.; Schmid-Staiger, U.; Trösch, Walter
Zeitschriftenaufsatz
Journal Article

 

2001 A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect
Degen, J.; Uebele, A.; Retze, A.; Schmid-Staiger, U.; Trösch, Walter
Zeitschriftenaufsatz
Journal Article

 

2000 Verbesserter Abbau von kommunalen Klärschlämmen in einer zweistufigen Hochlast-Vergärungsanlage
Kempter, B.; Schmid-Staiger, U.; Trösch, Walter
Zeitschriftenaufsatz
Journal Article

 

1999 One and two-stage digestion of solid organic waste
Schober, G.; Schäfer, J.; Schmid Staiger, U.; Trösch, Walter
Zeitschriftenaufsatz
Journal Article

 

1999 Verfahren zum mikrobiellen Abbau organisch belasteter Substrate
Schwarting, N.; Schmid-Staiger, Ulrike; Trösch, W.
Patent

 

1998 Verfahren zum Vergaeren von Klaerschlamm
Schaefer, J.; Trösch, W.; Schmid-Staiger, Ulrike
Patent

 

 

Diese Liste ist ein Auszug aus der Publikationsplattform Fraunhofer-Publica

 

This list has been generated from the publication platform Fraunhofer-Publica

Economic large-scale cultivation of microalgae

In order to keep the investment costs as low as possible the assigned bioreactor should both be economically manufactured and to be installed without high expenditure. The "flat-panel airlift photobioreactor" developed at Fraunhofer IGB fulfills these criteria and is suitable therefore for the large-scale cultivation of micro algae.

The operating costs are determined by the productivity of the selected algal species. Therefore, the efficiency of the transformation of solar radiation energy into biomass must be as high as possible. The energetic expenditure for operation of the plant has to be kept small.

With the development of algal cultivation systems and processes therefore the following parameters must be considered:

  • Availability of light for the cells (light entry of appropriate intensity and even distribution to all cells)
  • Mixing (algal cells are partly very sensitive to shearing forces)
  • CO2 supply and discharge of growth-limiting oxygen
  • Temperature control.

The processing costs depend on the one hand on the respective product and the purity requirements, on the other hand on the cell density. A high cell densitiy with at the same time high product concentration decreases the processing costs substantially and must be therefore a goal of each reactor development.

Proceeds of algal biomass or their products is determined by their targeted application. High value products, which can be used in cosmetics, pharmacy and nutrition, will faster lead to an economical production than the use of simple algal biomass as feed additive or fertilizers. Coupling of high value and biomass production, whereby first the valuable material is extracted and the remaining biomass additionally can be marketed, is surely the most meaningful use in the sense of ecological and economic sustainability.

Why microalgae?

Grüne Mikroalgen
Grüne Mikroalgen unter dem Mikroskop.
Haematocyst mit rotem Farbstoff Astaxanthin.

As a regenerative source of raw material microalgae produce a large number of valuable substances – CO2-neutrally. Due to their ability of photosynthesis, where carbon dioxide from the atmosphere is bound with the help of sunlight, they can contribute to the reduction of CO2 in the atmosphere. Since algae need only water and mineral salts for their growth besides light and CO2, they help to decrease the phosphate and nitrate pollution of waters.

Far over 100,000 species of microalgae offer a huge biotechnological and economic potential. These organisms supply a variety of valuable substances, which are suitable for purposes of nutrition, animal feeding, pharmacy, cosmetics, as well as technical applications (e. g. heavy metal adsorption). Substances of economic interest for example are lipids, sterols, phycobiliproteins or antioxidants such as tocopherol (vitamin E), vitamin C, carotinoids, polyunsaturated fatty acids and the red pigment astaxanthin.

Furthermore, residual algal biomass can be used for regenerative energy production (biogas).

Recurring event

EIT Food Online Course

Algae Biotechnology

Techniques and opportunities for the sustainable bioeconomy

 

Level: Professionals

 

Aim of the course is to provide introductory training and theory in algal biology, culturing, growth and biotechnology under laboratory and small scale pilot facilities.

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