Strategies for avoiding foam
We are currently investigating various methods to avoid foam formation during fermentative MEL production. For CL, a fraction with a high cellobiose lipid concentration was collected by continuously separating the foam produced during fermentation. If this foam fraction is purified directly, only seven percent of the solvent quantity is required for the extraction of the cellobiose lipids, compared to conventional purification of the entire reactor content.
Software-assisted process optimization
The individual process steps are evaluated by means of a life cycle analysis and a techno-economic assessment during the course of development. With the aid of these assessments, process bottlenecks are identified and validated experimentally.. The findings obtained serve to continuously improve the economy and ecology of the overall process.
In parallel, we are researching genomes and transcriptomes of promising production strains using next-generation sequencing methods to elucidate the biosurfactant metabolism and its regulatory mechanisms and to change the product spectrum and productivity of the strains using metabolic engineering.