Array technologies

The basic principle of a microarray or biochip is that DNA or protein molecules are fixed in an ordered fashion i.e. a defined sequence onto a solid support. For analyzing genes or genomes for example, complex samples are isolated from cells or tissues of interest, labeled and investigated using such a biochip.

Biochips enable the researcher to look at thousands of genes or proteins in parallel in a single experiment. The individual DNA or protein probes act as sensors to gain information about transcription, expression or mutations of the respective gene.

Indications

  • Sepsis
  • Respiratory diseases
  • Skin infections
  • Resistence detection

Customer-specific DNA microarrays

Automated printing of microarrays.
Automated printing of microarrays.

The Fraunhofer IGB develops tailor-made microarrays for corporate customers, hospitals and research institutions to address a wide variety of applications.

The existing infrastructure makes it possible to develop customized microarrays from the probe design through to their immobilization by contact printing methods.

Reference projects

STD-Array – Identification of pathogens of sexually transmitted diseases with highly parallel diagnostics using DNA microarray

 

On behalf of Immundiagnostik AG Bensheim, Fraunhofer IGB is developing multiplex PCRs as a basis for DNA-based microarrays for highly parallel diagnostics of sexually transmitted infectious diseases. Automated processing of samples should enable simplified and cost-effective diagnostics in a point-of-care test.

FYI-Chip – Detection of human fungal pathogens using a lab-on-a-chip device

 

FYI-Chip: Development of a fully integrated lab-on-a-chip system for the rapid identification of fungal infections.

Molecular diagnostics: Chip for diagnosing sepsis

 

One focus of molecular diagnostics at the Fraunhofer IGB is the development of microarray-based diagnostics. DNA chips for the classification of mammary carcinoma, for example, enable personalized and efficient therapy and prognosis. In addition, using molecular-genetic methods, we are developing improved diagnostic arrays for non-Hodgkin lymphoma. Another field of molecular diagnostic activity at the Fraunhofer IGB is the chip-based diagnosis of infections, which, according to the World Health Organization, are among the most frequent causes of death.

FunPath – Genomic Approaches to Unravel the Molecular Mechanisms of Pathogenicity in the Human Fungal Pathogen Candida glabrata

 

 

Duration: February 2007 – July.2010

EUropean RESistance FUNgal – EURESFUN

 

The project aims to the elucidation of resistance mechanisms in human fungal pathogens resulting in a DNA array for detection of resistances.

 

Duration: December 2005 – November 2008

Diagnostics for individualized therapy of breast cancer

 

Mammary carcinoma – malignant cell proliferation in the breast – is believed to be the most widespread form of cancer among women in Germany. In industrialized countries one in ten women will suffer from breast cancer during her lifetime. With 45,000 incidences and 18,000 mortalities per year in Germany, not only is the disease of great concern to patients but is also a significant factor in health care expenditure.