Cell-based assays (GLP)

Services

  • Fast and easy qualitative and quantitative detection of pyrogens e.g. in medical devices, such as surgical instruments, catheters, implants and also intravenously administered drugs.
  • Screening for new agonists and antagonists of the immune receptors
  • Detection of individual ligands or combination of ligands with isolated PRRs

Good laboratory practice (GLP) test facility category 9:
Cell-based test systems for the determination of biological parameters

Several non-clinical tests are running at our category 9 GLP unit “Cell-based test systems for the determination of biological parameters” to support R&D projects that investigate biological parameters of samples using cell-based assays.

Examples are:

  • Testing of bioactivity, cytotoxicity and immunogenicity of compounds using immune receptor-based assays
  • Screening of TLR agonists/antagonists
  • Testing of antimicrobial properties of substances or surfaces
  • Detection of pyrogens and microbial residues (pathogen-associated microbial patterns, PAMPs)
  • Detection of viruses

Reference projects

Screening for new immunmodulators – Discovery and Delivery of PRR antagonists and agonists to regulate innate immune reaction

The receptors of the innate immune system recognize not only conserved molecular patterns of infectious pathogens, but also isolated chemical structures (PAMPs, pathogen-associated molecular patterns), and are referred to as pattern recognition receptors (PRRs).

 

Duration: November 2012 – October 2015

Publications

  1. Burger-Kentischer, A., Abele, I. S., Finkelmeier, D., Wiesmuller, K. H., and Rupp, S. (2010): A new cell-based innate immune receptor assay for the examination of receptor activity, ligand specificity, signalling pathways and the detection of pyrogens, J Immunol Methods, 358: 93-103 (2010).